Endocrine Abstracts

ObjectiveThe aim of this investigation is to study the redox status of osteoblasts in response to glucose and bacterial lipopolysaccharide (LPS) and their response to minocycline, in a simulated diabetogenic model, using the steroid marker 5 alpha- dihydrotestosterone (DHT)MethodMonolayer cultures of MG63 osteoblasts were established in Eagle’s MEM incubated for 24 h with radiolabelled testosterone, effe...

ObjectiveThis study investigates the oxidative effects of tumour necrosis factor alpha (TNF) and glucose (G) in a cell culture model of osteoblasts and the modifying role of minocycline (M), using a steroid marker of wound healing (5 alpha- dihydrotestosterone: DHT). This model simulates chronic inflammation and hyperglycaemia, relevant to chronic inflammatory periodontal disease in uncontrolled diabetics and prospects of adjunctive management, using min...

Increased severity of periodontal disease has been reported in post-menopausal oestrogen deficient subjects, made worse by smoking. It was relevant to investigate this concept, using fibroblasts in culture and extrapolating the yields of androgen metabolites as an index of healing. The aim of this study is to establish the effects of nicotine (N), oestradiol (O) and the alkaline phosphatase inhibitor levamisole (L) on androgen metabolism in human gingival fibroblasts in this c...

Glucocorticoids modulate the effects of other hormones and also mediate cell function. Dexamethasone, in common with androgen metabolites, has been shown to increase alkaline phosphatase activity and bone specific marker proteins The aim of this investigation is to establish an androgen responsive element to dexamethasone as an index of regenerative potential in the periodontium, using the alkaline phosphatase inhibitor levamisole (L). 4 cell lines of oral periosteal fibroblas...

Increased severity of periodontal disease has been reported in post-menopausal oestrogen deficient subjects, made worse by smoking. It was relevant to investigate this concept, using fibroblasts in culture and extrapolating the yields of androgen metabolites as an index of healing. The aim of this study is to establish the effects of nicotine (N), oestradiol (O) and the alkaline phosphatase inhibitor levamisole (L) on androgen metabolism in human gingival fibroblasts in this c...

The aim of this investigation is to study androgen metabolism in human gingival fibroblasts exposed to a hyperglycaemic environment and their response to insulin, the antagonist glucagon and nicotine; this may be extrapolated to their metabolic responses in vivo, in uncontrolled diabetes (obtained local Ethical Committee approval). Duplicate incubations of four cell lines of monolayer cultures of fibroblasts were performed in Eagle's MEM with radiolabelled testosterone as subs...

Nicotine has been reported to have detrimental effects on fibroblasts while androgen metabolites and oestradiol are potentially anabolic. The aim of this investigation is to study the effects of oestradiol and nicotine on androgen metabolism as an index of healing. Monolayer cultures of human gingival fibroblasts (Approved by the local Ethics Committee) were established in Eagle's MEM with 14C-testosterone / 14C-4-androstenedione (4-A) as substrates. (Nicotine (N:1,50 microgra...

The anti-convulsant phenytoin stimulates matrix synthesis in inflamed gingivae. Oestrogen and androgen metabolites have anabolic effects on target tissue. The aim of this investigation is to study the effects of phenytoin on androgen metabolism in human gingival fibroblasts in response to oestradiol and the anti-androgen finasteride (approved by the local Ethics Committee). Monolayer cultures of human gingival fibroblasts were established in Eagle's MEM with 14C-testosterone a...

Alkaline phosphatase (ALP) is an important enzyme in the turnover of connective tissue and bone matrix. In view of the anabolic effects of the physiologically active androgen metabolite 5alpha dihydrotestosterone (DHT), it was relevant to investigate the effects of ALP and its inhibitor levamisole (L) on androgen metabolism in oral periosteal fibroblasts. Four cell-lines of oral periosteal fibroblasts (approved by the local Ethics Committee)were incubated in duplicate in Eagle...